This research focused on fracture and fatigue response of carbon/epoxy laminates modified with electrospun nylon 66 nanofibers. For this purpose, AS4/8552 composite laminates interleaved by nanofibrous mat were considered under mode-I quasi-static and fatigue loading conditions. According to test outcomes, the fracture toughness increased about 133% under quasi-static tests. On the other hand, the plotted fatigue curves showed that the crack growth rate significantly decreased in modified samples and the threshold energy release rate enhanced about 128%. Finally, scanning electron microscopy (SEM) was also conducted to investigate the damage mechanisms.