Chronic wounds are susceptible to infection and difficult to heal due to the complex healing process and terrible microenvironment. In order to overcome this problem, TOLs-CS/PEO nanofibrous film was successfully electrospun. The 6%(v/v) TOLs incorporation into CS/PEO nanofibrous film could improve the morphology and structure of nanofibers, weaken the interactions among macromolecules and rebalance the relationship between hydrophilicity and hydrophobicity of pore surface. Water vapor transmission rate and simulated tissue fluid absorption of films were optimized to reach 2193 g m(-2)d(-1)and 1676% so that to obtain the excellent moisture retention. The Young's modulus and elongation at break of films in wet state were 114.69 kPa and 72.04%, respectively, causing the mechanical characteristics similar to human skin. The film could effectively block more than 98% of bacteria and inhibit bacteria growth besides possessing procoagulant property and suitable biodegradability. These findings demonstrate that TOLs-CS/PEO nanofibrous film can construct a microenvironment suitable for wound healing.