In the past few years graphene quantum dots (GQDs) have been used as a signaling agent for medical diagnosis. They can be modified and labeled with different macromolecules to give them potential to be attached to a specific target. Herein GQDs were labeled with an antibody which is specific for cancer-derived exosomes, isolated from blood serum by using a specialized PCL-gelatin core-shell NFM. This membrane showed excellent sensitivity for isolating exosomes from a complex mixture such as serum, and the GQD-antibody complex detected the isolated exosomes with great sensitivity. The final results allow this method to be considered as one that can be used to quantify the concentration of a desired analyte in a mixture.