A highly porous nonwoven thermoplastic polyurethane (TPU)/Polypropylene (PP) triboelectric nanogenerator (N-TENG) was developed. To fabricate the triboelectric layers, the TPU nanofiber was directly electrospun onto the nonwoven PP at different basis weights (15, 30, and 50 g/m2). The surface morphologies and porosities of the nonwoven PP and TPU nanofiber mats were characterized by field-emission scanning electron microscopy and porosimetry. The triboelectric performance of the nonwoven TPU/PP based TENG was found to improve with an increase in the basis weight of nonwoven PP. The maximum output voltage and current of the TPU/PP N-TENG with 50% PP basis weight reached 110.18 +/- 6.06 V and 7.28 +/- 0.67 mu A, respectively, due to high air volume of nonwoven without spacers. In order to demonstrate its practical application as a generator, a TPU/PP N-TENG-attached insole for footwear was fabricated. The N-TENG was used as a power source to turn on 57 light-emitting diodes through human-walking, without any charging system. Thus, owing to its excellent energy-conversion performance, simple fabrication process, and low cost, the breathable and wearable nonwoven fiber-based TENG is suitable for large-scale production, to be used in wearable devices.