The aim of this research is to investigate the possibility of barrier effect of poly(vinyl alcohol) (PVA)/montmorillonite (MMT) nanofiber, against the UV radiation in different electrospinning situations. For this purpose, nanofibers with different weight ratio of PVA and nanoclay (MMT) were electrospun under different circumstances, and the amount of protect that is created by the nanofiber web against UV radiation by examining the rate of degradation of methylene blue dye that was protected by the same web was estimated. The UV-vis spectrometry was used for the analysis of methylene blue color. In order to investigate the morphology and miscibility of PVA-MMT composite nanofiber, X-ray diffraction, scanning election microscope, and Fourier transform infrared spectrometer have been used. Thermal gravimetric analysis is used to investigate and explore how nanofibers behave against heat.