There is a growing need to develop strategies capable of engineering the anisotropic cartilaginous fibrous network in vitro and consequently overcome the anatomical and functional restrictions of the standard medical procedures used for cartilage regeneration. In this work, we suggest a fabrication procedure to build 3D anisotropic multi-layered fibrous scaffolds. Polycaprolactone (PCL) was used as bulk material for the different electrospun layers (horizontally, randomly and vertically aligned) that were assembled and then structurally maintained by a biocompatible graphene-oxide-collagen (GO-collagen) microporous network. To validate the resourcefulness of the technique, four PCL-GO-collagen scaffolds with different anisotropic properties were produced and characterized by analysing their depth dependent morphological and mechanical properties.