This study proposes a novel design of a laboratory built static collector using the 3D printing technology. This new collector produces aligned-to-random nanofibers in nanofibrous scaffold through electrospinning process. A design of experiment (DOE), based on response surface, analyzes the effect of the main process parameters; concentration, voltage, and distance; on the responses diameter and orientation. A quantifying approach has been used to investigate the orientation of nanofibers in the produced patterned scaffold through Fourier transforms method. The obtained results have proven a good potential to be used in tissue engineering application, especially for cells requiring specific guidance. (c) 2018 Wiley Periodicals, Inc. J Biomed Mater Res Part A: 106A: 2963-2972, 2018.