Polymer nanofibers, with their specific set of material properties, are favorable for many applications in biomedical engineering (scaffold, stent, or tissue engineering). This application, however, requires the ability to control the manufacturing process together with organization and orientation of the deposited fiber. Electrospinning device parameters leading to a stable fiber extrusion were already found, together with the influence of the individual working parameters on the fiber properties. It was also found that fiber steering via the external electrostatic field, created by auxiliary electrodes, has very limited steering capabilities. In this article, electrodynamic steering of the electrospun polymer nanofiber is discussed, with focus on the macroscopic fiber deposition pattern and microscopic fiber alignment and straightness. Different electric field distributions are examined, and the corresponding fiber collection patterns are demonstrated on a series of experiments. Finally, a mathematical model of a discretized fiber is created. Matching the simulation and experimental results allows for the determination of unknown fiber properties, like the structural damping coefficient or Stokes drag coefficient. Published by AIP Publishing.