The aim of this study was an investigation on the proliferation rate of normal human epidermal keratinocytes (NHEK) on the cross-linked and uncross-linked fibrinogen/polycaprolactone (Fbg/PCL) nanofibers to determine a suitable scaffold for skin tissue engineering. Nanofibrous scaffolds were prepared by electrospinning of different weight ratios of Fbg to PCL and were analyzed as morphology, surface chemical properties and cytocompatibility by scanning electron microscopy (SEM), attenuated total reflectance-Fourier transform infrared (ATR-FTIR) spectroscopy and the 3-(4,5-dimethylthiazol-2-yl)-2,5-diphenyl tetrazolium bromide (MTT) assay, respectively. The diameters of the blended uncross-linked scaffolds were in the range of 124 +/- 43 nm-209 +/- 155 nm. Cross-linking of scaffolds with glutaraldehyde did not make a significant change in the diameter of blended scaffolds in 16 h. Crosslinking also improved the tensile strength and weight loss rate of scaffolds. However, cross-linking demonstrated an unfavorable effect on the attachment and proliferation of NIIEK cells. The proliferation study revealed that uncrosslinked scaffolds containing 50% and 70% Fbg provide a better environment for the growth of NHEK cells, and can be considered promising scaffolds in tissue engineering applications.