Starch nanofiber based hemostat was prepared by electrospinning process for biomedical applications. Bead free uniform starch nanofiber along with polycaprolactone polymer was produced using new solvent combination. The swelling of the PCL/starch mat was 240% higher compared to pristine PCL mat. The blood clotting time of the developed PCL/starch mat was 156 s and the contact angle was 30.8 degrees. The results suggest that the developed nanofibers exhibited good hemostatic potential with quick rate of clotting to control blood loss in traumatic injuries. (C) 2018 Elsevier B.V. All rights reserved.