Chitosan/nylon-6 nanofibres with antibacterial activity properties were prepared by electrospinning a chitosan/nylon-6 solution (containing medium molecular weight chitosan). Nanofibres with diameters in the range of 139-190 nm were obtained. The yield of low concentration chitosan/nylon-6 nanofibres was higher than that of high concentration chitosan/nylon-6 nanofibres. The affinity of chitosan toward lead was studied using a Pb(NO3)(2) solution containing Pb(II) ions as the heavy metal solution. The rejection of lead ions by the chitosan/nylon membranes was investigated under various chitosan concentrations. The optimum removal values for Pb(NO2)(3) and NaCl were found to be 74% and 71%, respectively. The nanofibres developed in this study exhibited an excellent antibacterial activity (95%) against Escherichia coli.