This study aimed to develop a recombinant human erythropoietin/aloe vera gel-releasing polyvinyl alcohol/chitosan wound dressing by wet-electrospinning method and investigate the synergistic effects of erythropoietin and aloe vera gel on the excisional wound healing. The polyvinyl alcohol/chitosan (4:1 (w/w)) solution was electrospun into an aloe vera gel-containing water coagulation bath and then coated with the recombinant human erythropoietin-loaded chitosan nanoparticles prepared via ionic gelation technique. The developed sponge-like film was able to release the erythropoietin and aloe vera gel for at least 7 and 1days, respectively. The dressing was non-toxic to L929 fibroblastic cell line and non-adherent to the wound bed with the contact angle of 67.93 degrees +/- 2.31 degrees. It also possessed the water-uptake capacity and water vapor transmission rate of 26.40%+/- 3.37% and 2584.00 +/- 144.67gm(-2), respectively. The in vivo study demonstrated the synergistic wound-healing effects of aloe vera gel and erythropoietin on the full-thickness excisional wounds of Wistar rats. The erythropoietin/aloe vera gel-releasing dressing had significantly (n=4, p<0.005) higher wound closure (92.96%+/- 10.09%) than the sterile gauze (73.89%+/- 2.61%), as the control group, after 2weeks. The differences observed between the wound closure percentages of the aloe vera gel-releasing dressing (85.93%+/- 6.46%) and the erythropoietin/aloe vera gel-releasing dressing and control group were not statistically significant.