In the present work, flexible porous carbon nanofibers (PCNFs) of 175 nm diameter were prepared by electrospinning process using resole-type liquefied biomass-based resin (RLBR) as precursor. The addition of defined amount of polyvinyl alcohol (PVA) is essential for the bead-free fibers forming. The final PCNFs were obtained after one-step carbonization of as-spun nanofibers, which presented abundant micropores (0.9 and 1.8 nm) and high specific surface area of 956 m(2)/g without any activation process. This indicates that the flexible RLBR-based PCNFs will be promising not only in adsorbent and catalyst support materials but also as materials in electrode capacitance application.