In this study, we synthesized a konjac glucomannan (KGM)/poly dopamine (PDA)/Oxaliplatin nanofiber membrane with efficient and controlled drug release properties by using electrospinning technology. The structure of the polymer network nanofiber membrane was characterized by Fourier transform infrared spectroscopy (FT-IR), X-ray diffraction (XRD), field emission scanning electron microscopy (SEM), and thermogravimetric analysis (TGA). KGM/PDA nanofiber membrane showed uniform sizes with an average diameter of 100-120 nm. The results revealed that the association of KGM and PDA to synthesize nanofiber membrane could be attributed to hydrogen bonds. Compared with KGM, the KGM/PDA nanofiber membrane exhibited higher moisture adsorption and drug release ability. Based on superior drug release ability, the KGM/PDA nanofibers provided a potential oxaliplatin carriers in application of drug release. This work contributed to a facile pathway on the construction of biomaterial nanofiber membrane by using polysaccharides. Scheme 1. Schematic presentation of the preparation of KGM/PDA nanofiber membrane and the use for drug release. [GRAPHICS] .