A small scale study was carried out to investigate the therapeutic efficacy cisplatin loaded poly-caprolactone/chitosan composite electrospun nanofibers for local chemotherapy of cervical cancers in mice. The prepared nanofibers had shown the sustained release pattern up to one month. Prepared nanofibers were found to have greater mucoadhesive strength. An orthotopic cervical cancer model was established by inducing the EAC cell lines in the vaginal mucosa at cervix region of the mice. Intravaginal administration of the cisplatin loaded nano fibers showed lesser % cell viability as compared to the plain drug. In vivo studies showed a better anti-tumour efficacy of prepared nanofibers in animals at 14th and 21st after the beginning of treatment. Therefore the technique of electrospinning provides a favourable approach for the targeted delivery of the anti-cancer drug via vaginal route against cervical cancer. (C) 2017 Elsevier B.V. All rights reserved.