This study was aimed at the synthesis and characterization of novel Titania nanorods by sol gel electrospinning technique. The physicochemical properties of the synthesized nanorods were determined by field emission scanning electron microscopy (FE-SEM), energy dispersive X-ray spectroscopy (EDX), transmission electron microscopy (TEM), and X-ray diffraction (XRD) pattern. To examine the in vitro cytotoxicity, mouse myoblast C2C12 cells were treated with different concentrations of as prepared TiO2 nanorods and the viability of cells was analyzed by Cell Counting Kit-8 assay at regular time intervals. The morphological features of the cells attached with nanorods were examined by Bio-SEM. Cytotoxicity experiments indicated that the mouse myoblast cells could attach to the TiO2 nanorods after being cultured. We observed that TiO2 nanorods could support cell adhesion and growth and guide spreading behavior of myoblasts. We conclude that the electrospun TiO2 nanorods scaffolds with unique morphology had excellent biocompatibility. Thus, the current work demonstrates that the as-synthesized TiO2 nanorods represent a promising biomaterial to be exploited for various tissue engineering applications. (C) 2012 Elsevier B.V. All rights reserved.