In this paper, polyvinylidene fluoride (PVDF) nanofibers have been suggested as a viable substrate for flexible and implantable electrochemical devices. PVDF electrospun nanofibers exhibit excellent mechanical properties, flexibility, chemical stability, and biocompatibility, making them a potential option in the development of implant fuel cells. This paper presents a membraneless hydrogen peroxide fuel cell that is fabricated to demonstrate the possibility of using these nanofibers as the substrate for electrochemical devices. An open circuit potential of 0.65 V was achieved for the cell fabricated using Prussian Blue (PB) as the cathode material and nickel and aluminium as the anode materials. The power produced by the cell was similar to 1 mW cm(-2) at 0.32 V. The results presented compare favourably with available power generators reviewed in the literature. Based on the proof of concept demonstration; PVDF electrospun nanofibers can be successfully used for implantable electrochemical devices such as bio-fuel cells and self-sustained point-of-care diagnostic systems.