This study focuses on the fabrication of poly(vinyl alcohol)/ poly(vinyl pyrrolidone)-Iodine nanofibers via electrospinning. Electrospun fibers were characterized by FT-IR, DSC and SEM techniques. DSC results indicated that the thermal stability of nanofibers were improved after the addition of chitosan and poloxamer 188. SEM images showed that the spongiform structure is much more compact and fibrous in the case of added chitosan, with an average fiber diameter of 374nm, whereas the addition of poloxamer 188 resulted in a more porous and beaded composition, with average fiber diameter of 489nm.