During wound healing, the contractile activity of myofibroblasts differentiated from fibroblasts in active transforming growth factor beta 1 (TGF beta 1) conditions has vital functions. In wound dressing biomaterials, it is crucial to mimic the extracellular matrix to deliver the right amount of TGFb1 in a spatiotemporally controlled manner. We report here, for the first time, a zero-order, sustained TGFb1 release from electrospun biomimetic nanofibers realizing optimal cell viability and myofibroblast differentiation capacity, confirmed by cell metabolic activity CCK assay, gene expression level through real-time PCR and protein expression level through immunochemical staining.