This research focused on the improvement of the physical properties of biopolyurethane (BPU) using cellulose nanowhisker (CNW). For the study, BPU was synthesized by one-shot polymerization from corn sugar-based polyol and methylene diiscocyanate (MDI). CNW was prepared via conventional acid hydrolysis of pulp using sulfuric acid. The synthesized BPU was mixed with the CNW and then electrospun. The addition of CNW improved simultaneously the hydrophilicity and the mechanical properties of BPU. The improved properties will give more opportunity to BPU for medical applications.