This study focused on the preparation of electrospun cross-linked double-network (DN) of agarose/polyacrylamide (PAAm) nanofibers. The agarose formed the first-network that was physical-linked by the agar helix bundles. After UV-irradiation, the chemically crosslinked PAAm was formed as the second network. The resulting cross-linked DN agarose/PAAm nanofibers were characterized by scanning electron microscopy (SEM), contact angle, attenuated total reflectance-Fourier transform infrared spectroscopy (ATR-FT-IR), thermogravimetric analysis (TGA), and tensile test. SEM analysis shows the agarose/PAAM nanofibers present with the thickness of 187 nm. Agarose/PAAm nanofibers were showing FT-IR spectral peaks at similar to 1660, 1590, and 1070 cm 21 indicating the presence of both agarose and polyacrylamide in the crosslinked DN Agarose/PAAm nanofiber sheet, it suggests the strong interaction and good compatibility between the two components. Agarose/PAAm nanofiber sheet was showing thermal stability close to the pure polyacrylamide. From the tensile test study, agarose/PAAm strength improved by 66.66% compared to the pure agarose. (C) 2015 Wiley Periodicals, Inc.