Controlling the architecture of electrospun fibers is one of the key issues in tissue engineering. This report describes a rapid and reproducible patterning method for the fabrication of an electrospun fibrous scaffold. The electrospun fibers were deposited on a patterned electrode. The patterned scaffold was fabricated using a thin insulating film between layers of this electrode. For a tissue engineering application, poly(lactic-co-glycolic acid) (PLGA), a biocompatible and biodegradable material, was electrospun. Fibroblast cells were cultured on the fibrous PLGA scaffold and the viability, morphology, and distribution of the cells were studied.