Nano-silver powders (n-Ag)/Anisodamine (AD)/poly (lactide-co-glycolide) (PLGA) were prepared by electrospinning. Field emission scanning electron microscope (ESEM) and Fourier transform infrared spectroscopy (FTIR) were applied for the investigation of the microscopic structure of the dressings as well as drug release, antibacterial and biological functions so that we could comprehensively evaluate the functionality of the dressings. The results show that the fibers exhibit a fully interconnected pore structure with narrow pore size distribution. And the diameters of electrospun fibers are influenced by the incorporation of AD. The result of drug release test proves that anisodamine can gradually release. It is also found that the n-Ag/AD/PLGA dressings exhibit a well antibacterial ability. The cell experimental results show that when the content of AD is from 1% to 5%, the dressings are non-toxic and contribute to the growth and proliferation of mouse L929 cells. Therefore, we conclude that the n-Ag/AD/PLGA dressing possesses good biocompatibility, antibacterial properties, and effectively release. It provides a new strategy for clinical treatment in diabetic foot infection.