Electrically conductive and antibacterial material was prepared via in situ chemical polymerization of polypyrrole on electrospun fibers of Eudragit L-100 and Eudragit L-100/polyethylene oxide, providing an "all-polymer" template for adsorption and removal of Staphylococcus aureus from surfaces. The optimization in the bactericidal activity of resulting wipes depends on type and concentration of oxidizing agent which affects the morphology and electrical properties of resulting material. We report the optimization provided by ferric chloride (oxidant) on bactericidal activity of wipes of Eudragit L-100/polypyrrole electrospun fibers. Promising results for S. aureus removal confirm the potential application of this polymeric material. (C) 2017 Elsevier Ltd. All rights reserved.