Traditional strategies to develop Au metal nanoparticle catalysts for glucose oxidation comprise Au nanoparticles (NPs) supported on electrode surfaces. In this work, the fabrication of a new material capable of acting as an abiotic anode for the electrooxidation of glucose was developed. The material is composed of electrospun carbon fibers containing gold nanoparticles formed insitu from a polyacrylonitrile (PAN) solution with HAuCl4. This approach allows the pre-reduction of the gold salt by PAN under mild conditions without the need for extra energy and leads to very stable carbon-gold bonding with well-dispersed AuNPs, not previously reported. The gold-modified carbon fibers (Au@CFs) were characterized by SEM, energy-dispersive X-ray spectroscopy, XRD, and electrochemical analysis. The Au@CF electrodes showed electrochemical activity toward glucose oxidation in alkaline media. Combined to a bilirubin oxidase modified biocathode (BOD@CFs), the resulting hybrid glucose biofuel cell showed open-circuit voltage and power density values of 0.75V and 65Wcm(-2), respectively, which remained intact after 3weeks.