In this study, a mat/membrane consisting of overlaid PVA/PA6-Cu(II) composite nanofibers was prepared via the electrospinning technique followed by coordination/chelation with Cu(11) ions; an enzyme of catalase (CAT) was then immobilized onto the PVA/PA6-Cu(II) nanofibrous membrane. The amount of immobilized catalase reached a high value of 64 +/- 4.6 mg/g, while the kinetic parameters (V-max and K-m) of enzyme were 3774 mu mol/mg.min and 41.13 mM, respectively. Furthermore, the thermal stability and storage stability of immobilized catalase were improved significantly. Thereafter, a plug-flow type of immobilized enzyme membrane reactor (IEMR) was assembled from the PVA/PA6-Cu(II) CAT membrane. With the increase of operational pressure from 0.02 to 0.2 MPa, the flux value of IEMR increased from 0.20 +/- 0.02 to 0.76 +/- 0.04 L/m(2).min, whereas the conversion ratio of H2O2 decreased slightly from 92 +/- 2.5% to 87 +/- 2.1%. After 5 repeating cycles, the production capacity of IEMR was merely decreased from 0.144 +/- 0.006 to 0.102 +/- 0.004 mol/m(2).min. These results indicated that the assembled IEMR possessed high productivity and excellent reusability, suggesting that the IEMR based on electrospun PVA/PA6-Cu(II) nanofibrous membrane might have great potential for various applications, particularly those related to environmental protection.