Biotin has been incorporated into poly lactic acid (PLA) electrospun fibers by either suspending biotin in the spinning dope or dissolving biotin in the spinning dope. Significant loading of biotin (10-18% w/w PLA) was achieved by both methods. Suspension of the biotin in the resulting fibers was mapped by Electron Probe Microanalysis (EPMA) via detection of the sulfur atom in the biotin molecule. Any crystallization of biotin in the fibers was measured with differential scanning calorimetry (DSC) and X-ray diffraction (XRD). Biotin incorporated via suspension in the spinning dope aggregated and to some extent crystallized in the resulting fibers. Biotin incorporated via dissolution in the spinning dope was uniformly dispersed in the PLA fibers and did not form measurable crystals. In both cases, biotin incorporated in the PLA fibers was accessible for subsequent binding with protein molecules.