Creating a facile method that can improve the stability of Prussian blue (PB) in neutral and weak alkaline has proved extremely challenging. This study responses to this challenge by designing and fabricating nanoporous Prussian blue-poly(vinylidene fluoride) (PB-PVDF) composite membranes via an electrospinning techique. Benefiting from the porous structure, the PB-PVDF sensor exhibits excellent electrocatalytic activity toward ascorbic acid (AA) with linear range of 8.0-1.5 mM and low detection limit down to 2.5 mu M, although the amount of PB in the fiber is low (3%). The results show that PB-PVDF porous composite nanofiber not only retain the excellent electrocatalytic properties of PB itself, but also improve the stability of PB, which may represent a significant step toward the broader application of PB. (C) 2015 Elsevier B.V. All rights reserved.