Environmental stress factors could lead to the excess generation of reactive oxygen species (ROS) that induces various forms of skin damage related to oxidative stress. Polyhydroxylated fullerene derivative C-60(OH)(n), acting as an effective agent for prevention of skin aging, is widely used in the lotion and sunscreens in the field of cosmetics, but rarely used in the masks. In this study, we prepared C-60(OH)(n)-loaded nanofibrous membranes to protect human keratinocyte cells from ROS-associated damage and suppress the elevation of intracellular ROS and Ca2+ along with the apoptotic cell death. Two FDA-approved biodegradable polymers, PLGA and PCL, have been used for making the electrospun nanofibers, with C-60(OH)(n) added to the polymers as an antioxidant. The nanofibrous membranes with good biocompatibility might be potentially applied in clinical practice to reduce skin aging. (C) 2017 Chinese Chemical Society and Institute of Materia Medica, Chinese Academy of Medical Sciences. Published by Elsevier B.V. All rights reserved.