Silver nanoparticles have inhibitory and bactericidal effects on a wide range of bacteria. Here, we report on the biosynthesis of silver nanoparticles using Pseudomonas aeruginosa isolated from soil environment. Silver bionanopartilces (Bio/Ag NPs) was synthesized using the bacterial filtrate in the presence of 1mM silver nitrate and the produced particles were characterized using the X-ray diffraction. The particles size was ranged from 50 to 70 nm. The antibacterial activity of the bionanopartilces was done on ten different pathogenic bacteria (P. aeruginosa, Staphylococcus aureus, Escherichia coli, Acinetobacter sp., Klebsiella pneumonia, Micrococcus sp., Staphylococcus epidermidis and candida sp.) using disc diffusion method. The toxicity of the particles was high when compared with the antibiotics, metronidazole, amoxicillin, vancomycin and chloramphenicol. Theprepared Poly (acrylonitrile-co-methyl methacrylate) was grafted with bionanosilver in the presence of P. aeruginos filtrate. Moreover, the polymer-bionanosilver complex was used for synthesis of bionanofiber using the electrospinning method. The diameter of the obtained bionanofibers ranged between 50 and 400 nm, as revealed by scanning electron microscopy. The morphology of the fabrics was investigated by scanning electron microscope (SEM), while their composition was measured by X-ray (EDX) and a good crystalline feature of silver metal was obtained. The activity of the bionanofiber pieces as antibacterial was examined on ten different pathogenic bacterial isolates compared with different eight antibiotics (Ampicillin, Kanamycin, Tetracycline, Chloramphenicol, Penicillin, Cephalosporins, Cefotoxin and Metronidazole). The bionanofiber gave different clear zones with different sizes with the ten pathogenic bacteria and it was observed that the product was mostly specific for S. aureus and E. coli.