Luminescent organic nanofibers were fabricated by electro-spinning the mixture of polar polymer polyacrylonitrile and rhodamine 6G dissolved in polar solvent dimethylformamide. SEM observations revealed that large volumes of nanofibers were successfully fabricated at the line velocity of 8 ms(-1) with constant diameter along the whole length of nanofibers. Fluorescence optical microscopy revealed that homogeneously luminescent nanofibers were deposited on a rotating cylinder electrode with reduced entanglement, opening a novel usage as detached separate nanofibers: when intentionally embedded in commercial textiles, they will function as, for example, forensic tracking owing to the invisibility to naked eyes. (C) 2017 WILEY-VCH Verlag GmbH & Co. KGaA, Weinheim