Nanoscale patterning of scaffolds provides broad surface for adhesion and differentiation of stem cells. As we know, the combination of tissue engineering with stem cells technology hold the key for regeneration of damaged tissues for example skeletal muscle tissues. On the other hand, the mechanical assessments of poly -caprolacton nanofibers determined the required features of biomedical scaffold for skeletal muscle tissue. In this study, skeletal muscle satellite cells as the main group of stem cells were cultivated on the electrospun poly -caprolacton nanofibers. Our results indicated that in comparison with tissue culture polystyrene, the nanoscale of scaffolds provided more induction to matured cells of skeletal muscle. Moreover, the immobilization of cells by collagen on poly -caprolacton nanofibers significantly improved the differential potency of satellite cells.