Alginate, a linear un-branched polysaccharide derived from seaweed has shown great potential as a cell scaffold for the regeneration of many tissues. However, alginate is not naturally enzymatically degraded in ionically crossliked alginate htdrogels exhibit a remarkably slow degradation rate, which is typically months to years for their complete removal from injection site. The ionizing irradiation degrades polysaccharides through the free radical-induced scission of glycosidic bonds of alginate. In this work, alginate/PEO nanofiber was irradiated Co-60 gamma-rays in the dose range of 50-300 kGy. This approach offers control over the degradation rate by varying the gamma-irradiation dose degree, as increasing the gamma-irradiation dose degree can increase the vulnerability of alginate nanofibers to hydrolysis under in vitro condition. And, the ability of electrospun a cell adhesive peptide Gly-Arg-Gly-AspS er-Pro (GRGDSP) modified alginate and unmodified alginate nanofibrous scaffolds to support human mesenchymal stem cell (hMSCs) attachment and spreading was greatly enhanced on the adhesion ligand-modified compared to unmodified nanofibers, demonstrating the initial promise of this electrospun polysaccharide material with defined nanoscale architecture and cell adhesive properties for tissue regeneration applications.