Objectives. Osteoporosis is one of the most common bone diseases in the world and results from an imbalance of bone cell functions. In the process of guided bone regeneration, osteoporosis weakens the bonding strength between scaffold and bone. Naringin is evidenced to be effective for the treatment of osteoporosis and bone resorption and the aim was to explore methods and benefits of its incorporation. Methods. In this study, naringin was incorporated in the electrospun nanoscaffold containingpoly(epsilon-caprolactone) (PCL) and poly(ethylene glycol)-block-poly(epsilon-caprolactone) (PEG-b-PCL). Results. The nanoscaffold demonstrated unchanged chemical structure, improved hydrophilicity, thinner and more uniform nanofibers by Fourier-transform infrared spectroscopy, contact angle measurement and scanning electron microscopy. The nanoscaffold also showed faster degradation rate and controlled-release of naringin. Osteoblast-nanoscaffold interactions were studied by the evaluation of adhesion, proliferation, differentiation of MC3T3-E1 osteoblasts and mineralization of ECM on the nanoscaffolds. Meanwhile, the response of osteoclasts to nanoscaffolds was evaluated in a mouse calvarialcritical size defect organ culture model. The osteoclasts around the bone defect were shown by tartrate resistant acid phosphatase staining. Significance. The results demonstrated that controlled-release naringin nanoscaffolds supported greater osteoblast adhesion, proliferation, differentiation, and mineralization and suppressed osteoclast formation. (C) 2014 Academy of Dental Materials. Published by Elsevier Ltd. All rights reserved.