Three dimensional(3D) aligned electrospun fibres have a promising potential application in biomedical areas, such as biosensors, controlled drug release and tissue engineering. However, the fabrication of these fibres with tuneable microarchitectures, porosity and overall morphology still has challenges due to electrospinning process. Thus, it is highly desired to develop novel, cost. effective and easily scalable fabrication methods for the 3D aligned electrospun fibres. Herein, we developed a facile yet effective method for the preparation of 3D poly(vinylidene fluoride)(PVDF) aligned fibres(3D AFs) via an improved electrospinning technique. The obtained 3D AFs showed controllable morphology, diameter and fiber density. Furthermore, the obtained 3D AFs showed excellent in vitro biocompatibility. Moreover, the as. prepared 3D AFs enhanced cellular activities and induced directional cell growth along the direction of nanofibre orientation, thereby providing an excellent cue for the anchorage and migration of human mesenchymal stem cells (hMSCs). More importantly, cell proliferation in the 3D AFs was found to be significantly higher than that on the nanofibre mats(NFMs). Combined with controllable morphology and structure, we anticipate that this finding greatly enhances the potential applications of these 3D AFs for therapeutically relevant 3D cell cultures, tissue engineering, diagnostics and other biomedical applications.