Electrospinning is a well-known method to obtain nanofibers and collagen is a very attractive material for biological applications due to its bioactive nature. However, the preservation of the natural structure of collagen at the end products due to the different production parameters is an open question. In this study the importance of the preparation temperature for CD measurements and the solvent effect that employed in the electrospinning process was discussed. The decrease of the preparation temperature by 100 increased the measured PP-II (folded) fraction ratio from 37% to 52.5% and the nanofibers that were obtained from acidic solvent scored 59% of PP-H fraction ratio. (C) 2014 Elsevier B.V. All rights reserved.