The present contribution reports the fabrication and characterization of silk sericin (SS) nanofibers via electrospinning. The function of solution concentration on morphological appearance and average diameter of the electrospun SS membranes was investigated by scanning electron microscopy. The structure and physical properties were observed by Fourier transform infrared spectroscopy (FT-IR), differential scanning calorimetry (DSC), and thermogravimetric analysis (TG). All beads were acquired in lower concentration of SS solution, 1.3-8.5 wt% of SS solutions, and beaded fibers were produced from 9.6, 11.7, 14.2, and 16.5 wt% of SS solution. The perfect nanofibers acquiring average diameters of 145 and 184 nm were fabricated from 20.9 to 22.9 wt% of SS solution, respectively. Results from FT-IR showed that in the as-spun fibers, SS was present in a random coil conformation, while after heat treatment, the molecular structure of SS was transformed into a beta-sheet containing structure. DSC and TG analysis illustrated that trifluoroacetic acid can be eliminated by heating. The availability of SS nanofibers introduces a new set of possible uses of these amazing fibers at a scale not explored before. New uses include small diameter fibers for cell proliferation purposes, nanocomposite reinforcing fibers for nanotechnology, wound dressing, scaffolds for tissue engineering, and other biomedical applications.