The acidified fullerene (C-60) was doped into the polyaniline as a conducting dopant, then the poly (L-lactic acid)/polyaniline/C-60 (PLA/PANI/C-60) was prepared by the method of emulsion polymerization blending. The structure and properties of PLA/PANI/C-60 were characterized. The PLA/PANI/C-60 fiber films and fiber tubes were fabricated by the electrospinning-weaving technology. The morphology, hydrophilicity and electrical properties of PLA/PANI/C-60 fibers were studied. The result indicated that the resistivity of PLA/PANI/C-60 films reached to the grade of 10(4) Omega.cm, which was a semiconductor. An electrical stimulation device outputting the square pulse wave signals was designed and assembled according to the principle of the 555 Timer to be used for electrically stimulating the osteoblast cells on the PLA/PANI/C-60 fiber mats. The PLA/ PANI/C-60 fiber tube had stable shape and increasing hydrophilicity, so the PLA/PANI/C-60 fiber mat enhanced the adhesion, growth and proliferation of the osteoblast cells under the stimulation of 18 mu A/l Hz/500 ms signals when it was applied to be the electrical stimulation location material and the host matrix for the cell culture. C-60 could promote the chain growth of aniline oligomers during the emulsion polymerization, and improve the polymerization degree of polyaniline. The PLA/PANI/C-60 had the decreasing content of aniline oligomers and less poisonous effect on the osteoblast cells during the electrically stimulating. The appropriate electrical stimulation could enhance the differentiation of the growth factor to osteoblast and promote the growth and reproduction of osteoblasts with the PLA/PANI/C-60 mats as the concentrating electrical stimulation material.