The process of electrospinning can be affected by various parameters, leading to as-prepared nanofibers with different morphology and properties. In order to explore the impact of DC(+) high-voltage position on the resultant nanofibers, two setups with DC(+) high-voltage individually tethered to the needle (S-1) and the collecting plate (S-2) were fabricated. Nanofibers produced by both setups under the same conditions were examined to distinguish their differences in morphology and electrostatic properties. It was found that the nanofibers with positive surface potential produced by the S-1 setup have a larger surface coverage and porosity, smaller average diameter, and wider distribution of diameters. Furthermore, the differences between both setups in the trajectory of flying jets and the distribution of electric field intensity were studied. The results showed that the volume charge density (VCD) of the flying jets plays a crucial role in determining the morphology and electrostatic properties of the resultant nanofibers. The relationship between the position of DC(+) high-voltage and the VCD of flying jets was then discussed, which could develop a better understanding of the process of electrospinning and deliver more accurate control over the production of various functional nanofibers. (C) 2016 Wiley Periodicals, Inc.