The authors developed a modified collector that formed well-aligned poly(2-acrylamido-2-methylpropanesulphonic acid) (PAMPS)/poly(N-isopropylacrylamide) (PNIPAAm) nanofibres with no bead defects. Fibre morphologies on the aluminium plate collector and glass sheet collector were given by scanning electron microscope. The effects of electrospinning time on the ordered degree and the diameter of nanofibres were investigated. Results showed that the ordered degree of nanofibres was not obviously affected by the electrospinning time. Nanofibres' average diameter decreased from 282.92 to 103.56 nm with decrease of weight ratio of N,N-dimethylformamide (DMF) in electrospun solution. The range of diameter distribution narrowed as DMF concentration in electrospun solution decreased.