In the present study pure hyaluronic acid (HA) nanofibers were successfully fabricated at ambient temperature by electrospinning HA powder was dissolved in a combination solution of deionized water formic acid and DMF The effect of the electrospinning solvent on electrospinnability was investigated in detail the effect of the electric field between the needle capillary and a modified collector on the deposition of the charged fibers was discussed The addition of formic acid greatly improved the electrospinnability of HA solution pure HA nanofibers with a mean diameter below 100 nm were fabricated under the optimal condition (C) 2010 Elsevier Ltd All rights reserved