Electrospinning was used to prepare the nanofibrous membrane (NFM) of the composite comprising poly(vinylidene fluoride) and poly(aminophenylboronic acid) (PVdF/PAPBA-NFM). The PVdF/PAPBA-NFM displayed an excellent linear response to the detection of glucose for the concentration range of I to 15 mM with a response time of less than 6 s. Further experiments on amperometric sensing of glucose were performed in the presence of interferents such as uric acid, ascorbic acid, acetaminophen, fructose, mannose, etc. using PVdF/PAPBA-NFM. The interferents did not. give significant overlapping current signal during the determination of glucose. Also, PVdF/PAPBA-NFM possesses better reproducibility toward glucose detection and storage stability. (c) 2006 Elsevier Inc. All rights reserved.