Materials, such as biopolymers, can be applied to produce scaffolds as mechanical support for cell growth in regenerative medicine. Two examples are polycaprolactone (PCL) and poly (lactic-coglycolic acid) (PLGA), both used in this study to evaluate the behavior of umbilical cord-derived mesenchymal stem cells. The scaffolds were produced by the 3D printing technique using PCL as a polymer covered with PLGA fibers obtained by electrospinning. The cells were seeded in three concentrations: 8.5 x 10(3); 25.5 x 10(3) and 51.0 x 10(3) on the two surfaces of the scaffolds. With scanning electron microscopy (SEM), it was observed that the electrospun fibers were integrated into the 3D printed matrices. Confocal laser scanning microscopy and SEM confirmed the presence of attached cells and the lactate dehydrogenase release test showed the scaffolds were not cytotoxic. The cells were able to differentiate into osteogenic and chondrogenic lineages on the scaffolds. Mechanical test showed that the cells seeded on the 3D printed PCL matrices coated with PLGA electrospun nanofibers (3D + ES + SC) did not show significant difference in tensile modulus than the pure PCL matrix (3D) or PCL matrices coated with PLGA electrospun nanofibers (3D + ES). The combination of the two polymers facilitated the production of a support with greater mechanical stability due to the presence of the 3D printed PCL matrices fabricated by melted filaments and greater cell adhesion due to the PLGA fibers. The scaffolds are suitable for use in cell therapy and also for tissue regeneration purposes.