Three drug delivery systems viz. doxycycline hyclate entrapped poly (vinyl alcohol) (PVA) microspheres (DM), composite fibers of doxycycline hyclatepoly (3-hydroxybutyric acid) (PHB) electrospun fibers (DF), and doxycycline hyclateloaded poly (vinyl alcohol) (PVA) microspheres sandwiched between composite fibers consisting of poly (3-hydroxybutyric acid) (PHB) and doxycycline hyclate (DMF), were developed. The initial burst release studies revealed that DMF drug delivery system developed through suspension electrospinning possessed an optimal initial burst release for wound healing. The developed scaffolds will potentially function in mimicking the extracellular matrix and consecutively enhance healing through its optimal drug release thereby proving its mettle as potential wound dressings for skin regeneration.