In this study, electrospun poly(epsilon-caprolactone)/titanium dioxide nanocomposite membranes were fabricated and characterized. Fourier transform infrared analysis showed an increase in absorbance of vibrational peaks of poly(epsilon-caprolactone) as the titanium dioxide nanoparticle concentration was increased. From the X-ray diffraction spectra, it was observed that the incorporation of titanium dioxide nanoparticles does not affect the crystalline structure of the poly(epsilon-caprolactone); instead, the degree of crystallinity has been increased significantly. However, at higher concentrations of titanium dioxide nanoparticles, crystallinity was decreased to a lower value. Differential scanning calorimeter thermograms have also confirmed this tendency. Finally, efficacy of gentamicin-loaded poly(epsilon-caprolactone)/titanium dioxide nanocomposite membranes against a wound isolate of methicillin-resistant Staphylococcus aureus has been evaluated. The results demonstrated that poly(epsilon-caprolactone)/titanium dioxide nanocomposite membranes can synergistically act with gentamicin to inhibit the growth of methicillin-resistant S. aureus. [GRAPHICS] .