Forward osmosis (FO) is an emerging membrane separation process. However, the lack of high performance FO membrane hinders its wide application. In this study, self-sustained electrospun polyacrylonitrile (PAN) nanofiber supported polyamide (PA) thin film composite (PA/PAN-eTFC) membrane was developed, and evaluated for simulated tetracycline (TC) wastewater treatment. Specifically, a PAN nanofiber support was fabricated by electrospinning without any backing layer. The PAN nanofiber support was then laminated by a paper laminator. The laminated nanofiber support possessed good hydrophilicity and mechanical properties with water contact angle, stress and strain of 32.3 +/- 1.3 degrees, 13 +/- 0.77 MPa and 68 +/- 0.28%, respectively. Polyamide composited membrane formed thereon demonstrated a low structural parameter (S = 168 mu m), high permselectivity (A = 1.47 LMH bar-1, B = 0.278 LMH), and achieved over 57 LMH water flux using 2 M NaCl as draw solution. The PA/PAN-eTFC was successfully applied in a forward osmosis-membrane distillation (FO-MD) hybrid process for treatment of simulated TC wastewater and water production for the first time. TC rejection was higher than 99.9%, and 15-22% water recovery was obtained after 7 h running in the FO-MD hybrid process. Meanwhile, the PA/PAN-eTFC membrane exhibited a relatively long-term stable performance in the hybrid system. These results indicate the PA/PAN-eTFC is a promising FO membrane for wastewater treatment.