In recent years, the ability to produce nanofibrous patterned architectures by electrospinning has exposed a wide range of potential applications in biomedical and industrial fields. Directional alignment, controlled deposition, and density variation into the patterns are desirable for many applications such as tissue engineering scaffolds and micro/nano-electronic devices. In this study, we introduce a versatile method for fabrication of various kinds of nanofibrous deposition patterns with the help of microprocessor based control system for switching collector electrodes. By controlling the concurrent activation time of two adjacent electrodes, we demonstrated that amount of fibers going into the pattern can be adjusted and alignment in electrospun fibers can be obtained. We also revealed that the deposition density of electrospun fibers in different areas of patterned architectures can be varied. This advanced technique can have a significant impact in enhancing the technology of electrospinning and can help develop new applications in health sciences and industrial sectors. Copyright (C) 2015 John Wiley & Sons, Ltd.