Electrospinning is a technique far creating, continuous nanofibrous networks that can architecturally be similar to the structure. of extracellular matrix (ECM). However, the shrinkage of electrospun mats is unfavorable for the triggering of cell adhesion and further growth. In this work, electrospun PLGA nanofiber assemblies are utilized to create a scaffold. Aided by a polypropylene auxiliary supporter, the scaffold is able to maintain long-term integrity without dimensional shrinkage. This scaffold is also able to suspend in cell culture medium; hence, keratinocyte cells seeded on the scaffold are exposed to air as required in skin tissue, engineering. Experiments also Show that human skin keratinocytes can proliferate on the scaffold and infiltrate into the scaffold.