The effect of increasing honey concentrations from 10% to 30% within the Honey (H)/polyvinyl alcohol (P)/chitosan (CS) nanofibers was investigated. Changes in the electrospun nanofiber diameters, crystallinity, thermal behavior, porosity and antibacterial activity have been assessed using am, XRD, DSC, TGA, mercury porosimeter and viable cell count technique. The HPCS nanofibers were cross-linked and tested for their swelling abilities and degradation behavior. The mean diameter of HPCS nanofibers increased from 284 97 nm to 464 185 nm upon increasing the honey concentration from 10% to 30%. Irrespective the honey concentrations, the nanofibers have demonstrated enhanced porosity. Increasing the honey concentration resulted in a reduction in the swelling of the 1 h cross-linked HPCS nanofibers containing 10% and 30%H from 520% to 100%; respectively. Degradation after 30 days was reduced in the 3 h cross-linked HPCS nanofibers compared to the non-crosslinked HPCS nano fibers. Enhanced antibacterial activity was achieved against both Staphylococcus aureus and Escherichia colt upon increasing the honey concentration. Changing the honey concentration and, the extent of nanofiber crosslinking can be used to adjust different parameters of the HPCS nanofibers to suit their applications inwound healing and tissue engineering. (C) 2016 Elsevier B.V. All rights reserved.