Halloysite nanotubes (HNT) reinforced polylactic acid (PLA) nanocomposite fibers were produced using an electrospinning approach for biomedical applications. The PLA/HNT nanocomposite fibers were characterized using x-ray diffraction (XRD) and scanning electron microscopy (SEM). The various factors such as type of solvent, solution concentration. HNT loading and feed rate, affecting the electrospinning process, and the morphology of the nanofibers were investigated, and the optimum values for these parameters are suggested. The results indicated that the addition of dimethylformamide (DMF) to chloroform facilitated the electrospinning process because of the improvement in electrical conductivity and viscosity of the solution. Nanometer-sized fibers were obtained by the addition of HNT to PLA. HNT loadings had a significant effect on the morphology of the nanofibers. Bead-free fibers were produced at feed rates between 1 and 4 mL/h.